A role for the thiol isomerase protein ERP5 in platelet function.

A role for the thiol isomerase protein ERP5 in platelet function Running title: Thiol isomerase ERP5 and platelet function

HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY A role for the thiol isomerase protein ERP5 in platelet function

Formation and rearrangement of disulfide bonds during the correct folding of nascent proteins is modulated by a family of enzymes known as thiol isomerases, which include protein disulfide isomerase (PDI), endoplasmic reticulum protein 5 (ERP5), and ERP57. Recent evidence supports an alternative role for this family of proteins on the surface of cells, where they are involved in receptor remode...

Thiol isomerases in thrombus formation.

Protein disulfide isomerase (PDI), ERp5, and ERp57, among perhaps other thiol isomerases, are important for the initiation of thrombus formation. Using the laser injury thrombosis model in mice to induce in vivo arterial thrombus formation, it was shown that thrombus formation is associated with PDI secretion by platelets, that inhibition of PDI blocked platelet thrombus formation and fibrin ge...

The platelet-surface thiol isomerase enzyme ERp57 modulates platelet function

BACKGROUND Thiol isomerases are a family of endoplasmic reticulum enzymes which orchestrate redox-based modifications of protein disulphide bonds. Previous studies have identified important roles for the thiol isomerases PDI and ERp5 in the regulation of normal platelet function. AIM Recently, we demonstrated the presence of a further five thiol isomerases at the platelet surface. In this rep...

A humanized monoclonal antibody that inhibits platelet‐surface ERp72 reveals a role for ERp72 in thrombosis

Essentials ERp72 is a thiol isomerase enzyme. ERp72 levels increase at the platelet surface during platelet activation. We generated a humanized monoclonal antibody which blocks ERp72 enzyme activity (anti-ERp72). Anti-ERp72 inhibits platelet functional responses and thrombosis. SUMMARY Background Within the endoplasmic reticulum, thiol isomerase enzymes modulate the formation and rearrangeme...